Sensitivity comparison of nested RT-PCR and TaqMan real time PCR for intravitam diagnosis of rabies in animals from urine samples
نویسندگان
چکیده
منابع مشابه
Sensitivity Comparison of Nested RT-PCR with Immunofluorescence for Diagnosis of Rabies
In the present study sensitivity of diagnosis of rabies with Nested RT-PCR was compared with Immunofluorescence on 20 brain samples. RNA extraction was done using Qiazol method. Synthesis of cDNA was done using rabies specific primers. Nested set of primers were used to amplify highly conserved 762bp nucleoprotein gene region. Nested RT-PCR was able to diagnose rabies viral RNA in 12 out of 13 ...
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Human rhinoviruses (HRVs) are positive-stranded RNA viruses belonging to the Enterovirus genus in the family of Picornaviridae. Identification of the specific strain in HRV disease has been difficult because the traditional serological method is insensitive, labor intensive, and cumbersome. With the fast progress in molecular biological technique, more sensitive and faster molecular methods hav...
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In developing countries, brain tissues from rabies suspect animals are not always available for diagnosis for a variety of reasons, such as lack of transport to submit a carcass or the difficulty of removing an animal’s head or brain under field conditions. To enable diagnosis in such cases, there is a need for a reliable method, using an alternative non-neural tissue, which can be removed and ...
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ژورنال
عنوان ژورنال: Veterinary World
سال: 2013
ISSN: 0972-8988
DOI: 10.5455/vetworld.2013.189-192